Travel information centres in Sheffield and Meadowhall will close under transport plans to save £7.6m.
Paper timetables will also be scrapped, jobs lost and there will be reduced cleaning in interchanges and bus shelters in the plans by the South Yorkshire Passenger Transport Executive.
The Sheffield City Region Combined Authority agreed the budget proposals after local councils, which fund a levy to the executive, asked for it to be reduced by 10 per cent.
The facility on Arundel Gate will operate as a bus interchange only and be staffed by customer service assistants.
Passengers will have to use self-service kiosks and up to 50 jobs across South Yorkshire could be affected.
However, discretionary concessions for older people and disabled people will remain. The 70p child fare and rural, evening and Sunday bus services, funded by SYPTE, have also been protected.
Coun Steve Houghton, authority chairman, said: “We are pleased to be able to avoid reductions to local bus services and concessionary travel. However, it is with regret that the 10 per cent reduction to South Yorkshire’s public transport budget means there must be changes to other front line services.
“We realise that this means some customers may need to change the way they find information about bus, tram and train times, and how they buy tickets and apply for passes, and equality impact assessments have been carefully worked through as part of these plans.”